B.E.S.T. Labs, Inc.

Coliform Bacteria Drinking Water Sample Procedures
1. Wash hands thoroughly, do not open sample bottle until you are ready to proceed. Sample results are dependent on proper sampling technique.
2. Sample must be taken from a tap that is representative of the water distribution system, preferably from the sample tap located at or near the water pressure tank. If the pressure tank is not accessible, the sample will be collected from another water tap that is representative of the drinking water system.
3. Water tap must be free of aerators, strainers, hose attachments, mixing type faucets, and purification devices.
4. The COLD water tap must be used and the service line cleared before sampling by running the water for at least two minutes, or until the temperature changes.
5. Do not touch the inside of the sample bottle or cap.
6. Do not rinse sample container (white powder is a preservative).
7. Sterile sample containers must be filled to at least the 100 ml line so sample volume is sufficient to perform all required tests. Leave at least inch one air space to facilitate mixing of the sample by shaking.

The sample collector is responsible for properly packaging and returning the samples to the laboratory for analysis. Chill and protect from sunlight. All samples collected must be received by the laboratory within twenty-four hours. Upon delivery, the sample collector will relinquish custody of the samples to laboratory personnel.

Partial Chemicals and Nitrate/Nitrite Sampling Procedures
1. Do not open sample bottle until you are ready to sample.
2. Sample must be taken from a tap that is representative of the distribution system. Do not sample through Reverse Osmosis or Refrigerator filters unless the intent is to check them specifically.
3. The cold water tap must be used and the service line cleared before sampling by running water for at least two minutes, or until the temperature changes.
4. Fill 100 ml bottle with a one inch air space (or to the bottom of the neck). Sufficient room for mixing sample is necessary.
The sample collector is responsible for properly packaging and returning the samples to the laboratory for analysis. Chill and protect from sunlight. All samples collected must be received by the laboratory within twenty-four hours. Upon delivery, the sample collector will relinquish custody of the samples to laboratory personnel.